Bioactive properties of biostimulants obtained by enzymatic hydrolysis

Amino acid-based biostimulant is a commonly used category for those biostimulants that are obtained by hydrolysis of protein extracts. However, in addition to the content in L-Amino acids, they usually contain a complex matrix of several bioactive substances, including other organic-N compounds, mineral elements, carbohydrates, lipids, nucleotide derived metabolites and other compounds. This complex composition of bioactive components makes it difficult to ascribe a unique mode of action to the biostimulant. In order to identify the biostimulants properties from those merely due to nutritional effects, a 3- stage level study is proposed, considering agronomic, physiological and molecular assays assessments, with the objective to compile synergistic effects of a pool of ingredients and provide integration of different discipline approaches.