A DIGE Proteomic analysis of wheat flag leaf treated with Terra-Sorb Foliar, a free amino acid-based biostimulator.

The flag leaf is the most important source of carbohydrate during wheat kernel filling. Around a75% of all sugars stored in the kernel come from carbon fixed by this leaf. Terra-Sorb® foliar is anL-α-amino acid-based product from enzymatic hydrolysis for foliar application with a high ratio offree to total amino acids. Previous agronomical studies carried out on grassy, horticultural and treecrops have shown that application of Terra-Sorb ® increases plant photosynthetic activity andchlorophyll content, promotes rapid recovery from stress and improves fruit set.In this work we have undertaken a proteomic approach in order to explore molecular mechanismspotentially involved in the stimulating effect of Terra-Sorb® foliar on wheat yield when applied incommercial fields. A DIGE approach was used to compare the proteomes of treated vs controlplants in four biological replicates.